A globally applicable PCR-based detection and discrimination of BK and JC polyomaviruses.
Rev Inst Med Trop Sao Paulo
; 60: e47, 2018.
Article
em En
| MEDLINE
| ID: mdl-30231168
ABSTRACT
BKV and JCV belong to the Polyomaviridae family and are opportunistic agents associated with complications in immunocompromised individuals. Although a single screening assay for both viruses would be convenient, the diversity of BKV and JCV serotypes and genotypes is a methodological challenge. In this paper, we developed a PCR method able to detect and segregate BKV and JCV, despite these genetic discrepancies. A duplex semi-nested PCR (duplex snPCR) was designed to target a conserved region (639nt-1516nt) within the VP2 gene. In the first PCR, a primer set common to all BKV and JCV serotypes/ genotypes was used, followed by a semi-nested PCR with internal primers for BKV and JCV segregation. The limit of detection of the duplex snPCR was as low as 10 copies of BKV or JCV plasmids/µL. Specific products were observed when JCV and BKV plasmids were mixed in the same reaction. In field sample testing, the duplex snPCR detected and distinguished both viruses in different biological samples. Results were confirmed by Sanger's sequencing. The geographical complexity of BKV and JCV serotypes and genotypes imposes limits to a simple and universal method that could detect each virus. However, we describe here a sensitive and reliable PCR technique for BKV and JCV diagnosis that overcomes these limitations and could be universally applied.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA Viral
/
Reação em Cadeia da Polimerase
/
Vírus BK
/
Vírus JC
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Rev Inst Med Trop Sao Paulo
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Brasil