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A Label-Free Fluorescent DNA Calculator Based on Gold Nanoparticles for Sensitive Detection of ATP.
Zhang, Jingjing; Zhang, Shizhi; Niu, Chaoqun; Liu, Chen; Du, Jie; Chen, Yong.
Afiliação
  • Zhang J; State Key Laboratory of Marine Resource Utilization in South China Sea, College of Information Science & Technology, College of Materials and Chemical Engineering, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China. zhangjingjingaoxue@163.com.
  • Zhang S; State Key Laboratory of Marine Resource Utilization in South China Sea, College of Information Science & Technology, College of Materials and Chemical Engineering, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China. 13698954926@163.com.
  • Niu C; State Key Laboratory of Marine Resource Utilization in South China Sea, College of Information Science & Technology, College of Materials and Chemical Engineering, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China. chaoqunniu@163.com.
  • Liu C; State Key Laboratory of Marine Resource Utilization in South China Sea, College of Information Science & Technology, College of Materials and Chemical Engineering, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China. chen__liu@126.com.
  • Du J; State Key Laboratory of Marine Resource Utilization in South China Sea, College of Information Science & Technology, College of Materials and Chemical Engineering, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China. dujie@hainu.edu.cn.
  • Chen Y; State Key Laboratory of Marine Resource Utilization in South China Sea, College of Information Science & Technology, College of Materials and Chemical Engineering, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China. ychen2002@hainu.edu.cn.
Molecules ; 23(10)2018 Sep 29.
Article em En | MEDLINE | ID: mdl-30274237
ABSTRACT
Herein we described a deoxyribonucleic acid (DNA) calculator for sensitive detection of the determination of adenosine triphosphate (ATP) using gold nanoparticles (GNP) and PicoGreen fluorescence dye as signal transducer, and ATP and single-stranded DNA (DNA-M') as activators. The calculator-related performances including linearity, reaction time, logic gate, and selectivity were investigated, respectively. The results revealed that this oligonucleotide sensor was highly sensitive and selective. The detection range was 50⁻500 nmol/L (R² = 0.99391) and the detection limit was 46.5 nmol/L. The AND DNA calculator was successfully used for the ATP detection in human urine. Compared with other methods, this DNA calculator has the characteristics of being label-free, non-enzymic, simple, and highly sensitive.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: DNA de Cadeia Simples / Trifosfato de Adenosina / Nanopartículas Metálicas / Ouro Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: Molecules Assunto da revista: BIOLOGIA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: China
Texto completo
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: DNA de Cadeia Simples / Trifosfato de Adenosina / Nanopartículas Metálicas / Ouro Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: Molecules Assunto da revista: BIOLOGIA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: China