Your browser doesn't support javascript.
loading
Serially transplantable mammary epithelial cells express the Thy-1 antigen.
Lobo, Neethan Amit; Zabala, Maider; Qian, Dalong; Clarke, Michael F.
Afiliação
  • Lobo NA; Institute for Stem Cell Biology and Regenerative Medicine, School of Medicine, Stanford University, 265 Campus Drive, Stanford, CA, 94305, USA.
  • Zabala M; Cell and Molecular Biology Program, University of Michigan, Ann Arbor, MI, USA.
  • Qian D; Institute for Stem Cell Biology and Regenerative Medicine, School of Medicine, Stanford University, 265 Campus Drive, Stanford, CA, 94305, USA.
  • Clarke MF; Institute for Stem Cell Biology and Regenerative Medicine, School of Medicine, Stanford University, 265 Campus Drive, Stanford, CA, 94305, USA.
Breast Cancer Res ; 20(1): 121, 2018 10 10.
Article em En | MEDLINE | ID: mdl-30305179
BACKGROUND: Recent studies in murine mammary tissue have identified functionally distinct cell populations that may be isolated by surface phenotype or lineage tracing. Previous groups have shown that CD24medCD49fhigh cells enriched for long-lived mammary epithelial cells can be serially transplanted. METHODS: Flow cytometry-based enrichment of distinct phenotypic populations was assessed for their gene expression profiles and functional proliferative attributes in vitro and in vivo. RESULTS: Here, we show Thy-1 is differentially expressed in the CD24medCD49fhigh population, which allowed us to discern two functionally different populations. The Thy-1+CD24medCD49fhigh phenotype contained the majority of the serially transplantable epithelial cells. The Thy-1-CD24medCD49fhigh phenotype contains a rare progenitor population that is able to form primary mammary outgrowths with significantly decreased serial in vivo transplantation potential. CONCLUSIONS: Therefore, Thy-1 expression in the immature cell compartment is a useful tool to study the functional heterogeneity that drives mammary gland development and has implications for disease etiology.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Antígenos Thy-1 / Perfilação da Expressão Gênica / Células Epiteliais / Glândulas Mamárias Animais Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals / Female / Humans Idioma: En Revista: Breast Cancer Res Assunto da revista: NEOPLASIAS Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Antígenos Thy-1 / Perfilação da Expressão Gênica / Células Epiteliais / Glândulas Mamárias Animais Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals / Female / Humans Idioma: En Revista: Breast Cancer Res Assunto da revista: NEOPLASIAS Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Estados Unidos