Continuous Vector-free Gene Transfer with a Novel Microfluidic Chip and Nanoneedle Array.
Curr Drug Deliv
; 16(2): 164-170, 2019.
Article
em En
| MEDLINE
| ID: mdl-30332957
ABSTRACT
BACKGROUND:
Delivery of foreign cargoes into cells is of great value for bioengineering research and therapeutic applications.OBJECTIVE:
In this study, we proposed and established a carrier-free gene delivery platform utilizing staggered herringbone channel and silicon nanoneedle array, to achieve high-throughput in vitro gene transfection.METHODS:
With this microchip, fluidic micro vortices could be induced by the staggered-herringboneshaped grooves within the channel, which increased the contact frequency of the cells with the channel substrate. Transient disruptions on the cell membrane were well established by the nanoneedle array on the substrate.RESULT:
Compared to the conventional nanoneedle-based delivery system, proposed microfluidic chip achieved flow-through treatment with high gene transfection efficiency (higher than 20%) and ideal cell viability (higher than 95%).CONCLUSION:
It provides a continuous processing environment that can satisfy the transfection requirement of large amounts of biological molecules, showing high potential and promising prospect for both basic research and clinical application.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Técnicas de Transferência de Genes
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Nanoestruturas
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Dispositivos Lab-On-A-Chip
/
Agulhas
Limite:
Humans
Idioma:
En
Revista:
Curr Drug Deliv
Assunto da revista:
FARMACIA
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FARMACOLOGIA
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TERAPIA POR MEDICAMENTOS
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
China