Eco-Pap: The Ecological Modification of the Papanicolaou Stain for Sustainable Cervical Cancer Diagnosis.
Acta Cytol
; 63(1): 35-43, 2019.
Article
em En
| MEDLINE
| ID: mdl-30352442
ABSTRACT
OBJECTIVES:
To validate the ecological modification of the Papanicolaou stain (Eco-Pap) for the diagnosis of cervical cancer. STUDYDESIGN:
A prospective study was performed at the Mother and Child "San Bartolomé" Hospital, Lima, Peru. Reagent handling strategies were divided into three phases we used (1) Harris progressive hematoxylin (for nuclear staining), (2) a polychromatic solution (a mix of EA-36/Orange G-6 to suppress the use of several alcohol baths), and (3) direct mounting (with Entellan® solution). The cellular details were analyzed by the staining quality index, an external quality control, and the Bethesda System 2014.RESULTS:
We evaluated 72,901 cervical smears stained with the Eco-Pap. The validation of the Eco-Pap against the conventional Pap stain was optimal (κ = 0.89; 95% CI 0.87-0.92), showing a sensibility and specificity of 88.3% (95% CI 85.1-90.0) and 98.7% (95% CI 98-99.2), respectively. The Eco-Pap dramatically reduced the environmental pollution caused by 72 L of xylene, hydrochloric acid, and ammonia (6 L each) and mercury oxide.CONCLUSION:
The Eco-Pap is an innovative and efficient staining method reducing the use of toxic reagents with carcinogenic potential during cervical cancer screening by exfoliative cytology.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Coloração e Rotulagem
/
Esfregaço Vaginal
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Neoplasias do Colo do Útero
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Eliminação de Resíduos de Serviços de Saúde
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Poluentes Ambientais
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Poluição Ambiental
/
Teste de Papanicolaou
Tipo de estudo:
Diagnostic_studies
/
Observational_studies
/
Prevalence_studies
/
Prognostic_studies
/
Risk_factors_studies
Limite:
Female
/
Humans
País/Região como assunto:
America do sul
/
Peru
Idioma:
En
Revista:
Acta Cytol
Ano de publicação:
2019
Tipo de documento:
Article