Single-Molecule Kinetics in Living Cells.
Annu Rev Biochem
; 88: 635-659, 2019 06 20.
Article
em En
| MEDLINE
| ID: mdl-30359080
ABSTRACT
In the past decades, advances in microscopy have made it possible to study the dynamics of individual biomolecules in vitro and resolve intramolecular kinetics that would otherwise be hidden in ensemble averages. More recently, single-molecule methods have been used to image, localize, and track individually labeled macromolecules in the cytoplasm of living cells, allowing investigations of intermolecular kinetics under physiologically relevant conditions. In this review, we illuminate the particular advantages of single-molecule techniques when studying kinetics in living cells and discuss solutions to specific challenges associated with these methods.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Imagem Individual de Molécula
/
Microscopia de Fluorescência
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Annu Rev Biochem
Ano de publicação:
2019
Tipo de documento:
Article