Effect of 1α, 25-dihydroxyvitamin D3 on the osteogenic differentiation of human periodontal ligament stem cells and the underlying regulatory mechanism.
Int J Mol Med
; 43(1): 167-176, 2019 Jan.
Article
em En
| MEDLINE
| ID: mdl-30365053
ABSTRACT
1α, 25dihydroxyvitamin D3 (1,25D3), an active vitamin D metabolite, is a wellknown regulator of osteogenic differentiation. However, how 1,25D3 regulates osteogenic differentiation in human periodontal ligament stem cells (hPDLSCs) remains to be fully elucidated. The present study aimed to clarify this issue through wellcontrolled in vitro experiments. After hPDLSCs were treated with 1,25D3, immunofluorescence and western blotting were used to detect the expression of vitamin D receptor; Cell Counting Kit8 and western blotting were used to assay the cell proliferation ability. Alkaline phosphatase staining, Alizarin Red staining and western blotting were used to detect the osteogenic differentiation. It was found that treating hPDLSCs with 1,25D3 i) Inhibited cell proliferation; ii) promoted osteogenic differentiation; iii) upregulated the expression of transcriptional coactivator with PDZbinding motif (TAZ), an important downstream effector of Hippo signaling that has been demonstrated to be involved in the osteogenic differentiation of stem/progenitor cells; and iv) that cotreatment of TAZoverexpressing hPDLSCs with 1,25D3 synergistically stimulated the expression of osteogenic markers. These results suggested that the induction of osteogenic differentiation promoted by 1,25D3 in hPDLSCs involves, at least in part, the action of TAZ.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Osteogênese
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Ligamento Periodontal
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Células-Tronco
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Vitamina D
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Diferenciação Celular
Limite:
Adolescent
/
Child
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Humans
Idioma:
En
Revista:
Int J Mol Med
Assunto da revista:
BIOLOGIA MOLECULAR
/
GENETICA MEDICA
Ano de publicação:
2019
Tipo de documento:
Article