Gallic acid caused cultured mice TM4 Sertoli cells apoptosis and necrosis.
Asian-Australas J Anim Sci
; 32(5): 629-636, 2019 May.
Article
em En
| MEDLINE
| ID: mdl-30381745
ABSTRACT
OBJECTIVE:
The study was designed to determine the cytotoxic effect of gallic acid (GA), obtained by the hydrolysis of tannins, on mice TM4 Sertoli cells apoptosis.METHODS:
In the present study, non-tumorigenic mice TM4 Sertoli cells were treated with different concentrations of GA for 24 h. After treatment, cell viability was evaluated using WST-1, mitochondrial dysfunction, cells apoptosis and necrosis was detected using JC-1, Hoechst 33342 and propidium iodide staining. The expression levels of Cyclin B1, proliferating cell nuclear antigen (PCNA), Bcl-2-associated X protein (BAX), and Caspase-3 were also detected by quantitative real-time polymerase chain reaction and Western-blotting.RESULTS:
The results showed that 20 to 400 µM GA inhibited viability of TM4 Sertoli cells in a dose-dependent manner. Treatment with 400 µM GA significantly inhibited PCNA and Cyclin B1 expression, however up-regulated BAX and Caspase-3 expression, caused mitochondrial membrane depolarization, activated Caspase-3, and induced DNA damage, thus, markedly increased the numbers of dead cells.CONCLUSION:
Our findings showed that GA could disrupt mitochondrial function and caused TM4 cells to undergo apoptosis and necrosis.
Texto completo:
1
Base de dados:
MEDLINE
Idioma:
En
Revista:
Asian-Australas J Anim Sci
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
China