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miR-377-5p inhibits lung cancer cell proliferation, invasion, and cell cycle progression by targeting AKT1 signaling.
Wu, Han; Liu, Hai Yan; Liu, Wen Jie; Shi, Yong Li; Bao, Dawei.
Afiliação
  • Wu H; Department of Pathology, The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Huaian, China.
  • Liu HY; Department of Pathology, The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Huaian, China.
  • Liu WJ; Department of Pathology, The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Huaian, China.
  • Shi YL; Department of Pathology, The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Huaian, China.
  • Bao D; Department of Pathology, The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Huaian, China.
J Cell Biochem ; 120(5): 8120-8128, 2019 May.
Article em En | MEDLINE | ID: mdl-30485528
Lung carcinoma is the most common type of malignant tumors globally, and its molecular mechanisms remained unclear. With the aim to investigate the effects of microRNA (miR)-377-5p on the cell development, invasion, metastasis, and cycle of lung carcinoma, this study was performed. We evaluated miR-377-5p expression levels in lung cancer tissues and cell models. Cell viability, proliferation, migration, invasion abilities, and cell cycle distribution were measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, crystal violet, transwell, and flow cytometry assay. Furthermore, expression levels of protein kinase B α subunit (AKT1) and proteins related to cell cycle and epithelial-mesenchymal transition (EMT) were assessed using Western blot analysis and quantitative real-time polymerase chain reaction. These results suggested that miR-377-5p was downregulated in vivo and in cell models, and miR-377-5p overexpression inhibited cell viability, proliferation, migration, invasion, and induced cell-cycle arrest. In addition, as a target of miR-377-5p, AKT1 alleviated the decreases of cell viability, proliferation, migration, invasion, the S-phase cells, the expression of cyclin D1, fibronectin, and vimentin, as well as the increases of the G0/G1-phase cells, the expression of Foxo1, p27 kip1 , p21 Cip1 and E-cadherin when miR-377-5p overexpressed. In conclusion, miR-377-5p inhibited cell development and regulated cell cycle distribution and EMT by targeting AKT1, which provided a theoretical basis for further study of lung carcinoma therapeutics.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Revista: J Cell Biochem Ano de publicação: 2019 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Base de dados: MEDLINE Idioma: En Revista: J Cell Biochem Ano de publicação: 2019 Tipo de documento: Article País de afiliação: China