MiR381 regulates cell motility, growth and colony formation through PIK3CA in endometriosisassociated clear cell and endometrioid ovarian cancer.
Oncol Rep
; 40(6): 3734-3742, 2018 Dec.
Article
em En
| MEDLINE
| ID: mdl-30542723
ABSTRACT
Ovarian cancer is the one of the most lethal gynecological cancer types. MicroRNAs (miRs) are noncoding RNAs that modulate the translation of their target mRNAs via binding to a complementary sequence in the target 3' untranslated region, and the dysregulation of certain miRs has been demonstrated to contribute to cancer progression. In this regard, the current study extended our previous work and used nextgeneration sequencing data to search for upstream regulators of genetic alterations that are common in ovarian cancer, as well as the miRs that are involved in controlling the expression of these regulators. An miR prediction program was used to identify miR381 as an upstream regulator of phosphatidylinositol 3kinase catalytic subunit α (PIK3CA) in the context of ovarian cancer. Levels of miR381 were decreased in clear cell and endometrioid carcinoma ovarian cancer. Experimentally induced upregulation of miR381 led to a decrease in the level of PIK3CA in ovarian cancer cells. Furthermore, experimentally induced upregulation of miR381 inhibited the proliferation of ovarian cancer cells in vitro and their ability to form colonies and migrate. The observed decrease in miR381 in ovarian cancer could be reversed upon overexpression of the gene encoding the tumor suppressor homeobox D10. The current results highlight the role of miR381mediated regulation of PIK3CA in the development and progression of ovarian cancer and suggest that restoration of miR381 to normal levels in ovarian cancer cells may constitute a therapeutic strategy for patients.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Ovarianas
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Carcinoma Endometrioide
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Adenocarcinoma de Células Claras
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MicroRNAs
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Endometriose
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Classe I de Fosfatidilinositol 3-Quinases
Tipo de estudo:
Prognostic_studies
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Risk_factors_studies
Limite:
Adult
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Female
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Humans
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Middle aged
Idioma:
En
Revista:
Oncol Rep
Assunto da revista:
NEOPLASIAS
Ano de publicação:
2018
Tipo de documento:
Article