Development of a microdevice for facile analysis of theophylline in whole blood by a cloned enzyme donor immunoassay.
Lab Chip
; 19(2): 233-240, 2019 01 15.
Article
em En
| MEDLINE
| ID: mdl-30547178
ABSTRACT
We have developed a microdevice for therapeutic drug monitoring. In this device, dispensing of sample and reagent was accomplished by simple manual operation of a syringe. Moreover, for a simple and rapid measurement, we used cloned enzyme donor immunoassay as a detection principle. These features and the reagent that is enclosed in microdevice beforehand make it possible to complete the facile analysis. In this paper, our model analyte was 1,3-dimethylxanthine (theophylline), a kind of bronchodilator. The fluorescence measurement of theophylline in whole blood was achieved with the limit of detection of 0.73 µg mL-1. This microdevice provides rapid analysis (4 min), requires only a small volume of sample (2 µL) and features simple operation; hence, it is readily applicable to point of care testing.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Teofilina
/
Análise Química do Sangue
/
Técnicas Imunoenzimáticas
/
Monitoramento de Medicamentos
/
Técnicas Analíticas Microfluídicas
Tipo de estudo:
Diagnostic_studies
/
Guideline
/
Prognostic_studies
Limite:
Adult
/
Humans
/
Male
Idioma:
En
Revista:
Lab Chip
Assunto da revista:
BIOTECNOLOGIA
/
QUIMICA
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Japão