Low molecule weight fucoidan mitigates atherosclerosis in ApoE (-/-) mouse model through activating multiple signal pathway.

ABSTRACT

Atherosclerosis (AS) is a chronic inflammatory disease, and many factors are implicated in its progression. This work aims to study the preventive effects and the regulatory mechanism of low-molecular-weight fucoidan (LMWF), which is obtained from Saccharina japonica, on the development of AS. Serum biochemical indices and pathological analyses were determined via ELISA, hematoxylin and eosin (HE) and Oil Red O staining. These results indicated that LMWF ameliorated areas with atherosclerotic lesions and had a significant antioxidant effect. Anticoagulant assays showed that LMWF might serve as a potential anticoagulation drug for inhibiting the formation of atherothrombosis. Double immunofluorescence staining illustrated that LMWF inhibited both SMC proliferation and migration and macrophage formation and differentiation. A molecular biology experiment showed that LMWF exhibited an apparent regulatory effect on various signaling pathways. In conclusion, our results revealed that LMWF mitigated AS in the ApoE (-/-) mouse model by activating multiple signal pathways.