Production and characterisation of exopolysaccharide from Streptomyces carpaticus isolated from marine sediments in Egypt and its effect on breast and colon cell lines.
J Genet Eng Biotechnol
; 16(1): 23-28, 2018 Jun.
Article
em En
| MEDLINE
| ID: mdl-30647700
ABSTRACT
Twenty streptomycete strains were isolated from marine sediment samples collected from Nabq area, Sharm El-Sheikh, Red Sea Coast, Egypt. Four of them produce exopolysaccharides (EPS) showing marked in vitro antitumor activities. Morphological and cultural characteristics of the most significant strain (No. 3) were shown. Moreover, the sequence of this strain showed similarity with Streptomyces carpaticus. The results reveal that EPS produced by Streptomyces carpaticus No. 3 had high cytotoxicity reaching 51.7% and 59.1% against human tumor cells of breast and colon lines respectively. A chemical analysis of EPS indicated that the composing monosaccharides were galactouronic acid, glucose, xylose, galactose, mannose, and fructose with relative ratio of 311221 respectively, with an average molecular weight (Mw) 1.180 × 105â¯g/mol and of a number average molecular weight (Mn) 1.052 × 105â¯g/mol. Also the EPS contained uronic acid (0.5072%) and monosaccharide sulphates (21.753%).
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Base de dados:
MEDLINE
Idioma:
En
Revista:
J Genet Eng Biotechnol
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Egito