Light Regulates Plant Alternative Splicing through the Control of Transcriptional Elongation.
Mol Cell
; 73(5): 1066-1074.e3, 2019 03 07.
Article
em En
| MEDLINE
| ID: mdl-30661982
ABSTRACT
Light makes carbon fixation possible, allowing plant and animal life on Earth. We have previously shown that light regulates alternative splicing in plants. Light initiates a chloroplast retrograde signaling that regulates nuclear alternative splicing of a subset of Arabidopsis thaliana transcripts. Here, we show that light promotes RNA polymerase II (Pol II) elongation in the affected genes, whereas in darkness, elongation is lower. These changes in transcription are consistent with elongation causing the observed changes in alternative splicing, as revealed by different drug treatments and genetic evidence. The light control of splicing and elongation is abolished in an Arabidopsis mutant defective in the transcription factor IIS (TFIIS). We report that the chloroplast control of nuclear alternative splicing in plants responds to the kinetic coupling mechanism found in mammalian cells, providing unique evidence that coupling is important for a whole organism to respond to environmental cues.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Plantas Geneticamente Modificadas
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Arabidopsis
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Processamento Alternativo
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RNA de Plantas
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Regulação da Expressão Gênica de Plantas
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Elongação da Transcrição Genética
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Luz
Idioma:
En
Revista:
Mol Cell
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Argentina