Effect of Radiation on the Expression of CVD-related miRNAs, Inflammation and Endothelial Dysfunction of HUVECs.
Anticancer Res
; 39(2): 771-780, 2019 Feb.
Article
em En
| MEDLINE
| ID: mdl-30711956
ABSTRACT
BACKGROUND/AIM:
Radiotherapy (RT) can lead to cardiovascular disease (CVD). Evidence suggests that radiation modulates miRNA levels. Our purpose was to assess the acute response to radiation-induced modulation of the expression of miRNA-146a, miRNA-155, miRNA-221, and miRNA-222, inflammatory response and endothelial dysfunction on endothelial cells. MATERIALS ANDMETHODS:
Human umbilical vein endothelial cells (HUVECs) were exposed to 2 Gy RT, and intracellular levels of selected miRNAs were measured by real-time polymerase chain reaction at 2 and 24 h. Cytokine and adhesion molecule release were also assessed.RESULTS:
Results showed that 2 Gy significantly increased the expression of miRNA-221 and miRNA-222, and reduced the level of miRNA-155 after 2 h; whereas miRNA-146a and miRNA-155 were significantly overexpressed and miRNA-222 was significantly down-regulated at 24 h. Interleukin-8 and soluble vascular cell adhesion molecule 1 levels were not affected by the studied RT.CONCLUSION:
RT at 2 Gy modulated expression of selected miRNAs by endothelial cells after 2 and 24 h, which might be related to CVD development in patients who receive RT.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Endotélio Vascular
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Doenças Cardiovasculares
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MicroRNAs
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Células Endoteliais da Veia Umbilical Humana
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Inflamação
Limite:
Humans
Idioma:
En
Revista:
Anticancer Res
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Espanha