Flow cytometry for near-patient testing in premature neonates reveals variation in platelet function: a novel approach to guide platelet transfusion.
Pediatr Res
; 85(6): 874-884, 2019 05.
Article
em En
| MEDLINE
| ID: mdl-30742030
ABSTRACT
BACKGROUND:
Neonatal haemorrhaging is often co-observed with thrombocytopenia; however, no evidence of a causal relationship with low platelet count has been reported. Regardless, the administration of a platelet transfusion is often based upon this parameter. Accurate measurement of platelet function in small volumes of adult blood samples by flow cytometry is well established and we propose that the use of the same technology could provide complementary information to guide the administration of platelet transfusions in premature neonates.METHODS:
In 28 neonates born at 27-41 weeks gestation, platelet function after stimulation agonists was measured using fibrinogen binding and P-selectin expression (a marker of degranulation).RESULTS:
Platelets of neonates with gestation of ≤36 weeks (n = 20) showed reduced fibrinogen binding and degranulation with ADP, and reduced degranulation with CRP-XL. Degranulation Scores of 7837 ± 5548, 22,408 ± 5301 and 53,131 ± 12,102 (mean ± SEM) identified significant differences between three groups <29, 29-36 and >36 weeks gestation). Fibrinogen binding and degranulation responses to ADP were significantly reduced in suspected septic neonates (n = 6) and the Fibrinogen Binding scores clearly separated the septic and healthy group (88.2 ± 10.3 vs 38.6 ± 12.2, P = 0.03).CONCLUSIONS:
Flow cytometric measurement of platelet function identified clinically different neonatal groups and may eventually contribute to assessment of neonates requiring platelet transfusion.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Testes de Função Plaquetária
/
Recém-Nascido Prematuro
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Transfusão de Plaquetas
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Citometria de Fluxo
Tipo de estudo:
Prognostic_studies
Limite:
Female
/
Humans
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Male
/
Newborn
Idioma:
En
Revista:
Pediatr Res
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Reino Unido