Establishment of a comprehensive and high throughput serological algorithm for Zika virus diagnostic testing.
Diagn Microbiol Infect Dis
; 94(2): 140-146, 2019 Jun.
Article
em En
| MEDLINE
| ID: mdl-30744915
ABSTRACT
The previous serological algorithm for Zika virus (ZIKV) comprised screening by anti-ZIKV IgM capture ELISA (MAC-ELISA) for samples collected within 3 months postexposure or onset (MPEO). Samples positive by MAC-ELISA and samples collected beyond 3 MPEO were tested by the confirmatory plaque reduction neutralization test (PRNT), which proved laborious and time-consuming during the 2015 outbreak. Thus, we evaluated several ZIKV ELISAs to establish an anti-IgM and anti-IgG combination for use as a screening tool for all samples prior to PRNT confirmation. The MAC-ELISA or InBios-M in combination with the Euroimmun-G demonstrated sensitivities of 99.1% and 97.2%, respectively, and nonflavivirus specificity of 96.0%. Their cross-reactivities were 71.4% and 50.0%, respectively, for sera positive for Dengue virus antibodies. Due to near-perfect interrater agreement with PRNT and excellent detection of samples collected beyond 3 MPEO, these combinations were recommended as a screening protocol in a new high-throughput algorithm with special considerations for ZIKV diagnostics.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Algoritmos
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Testes Sorológicos
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Programas de Rastreamento
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Zika virus
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Infecção por Zika virus
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Anticorpos Antivirais
Tipo de estudo:
Diagnostic_studies
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Evaluation_studies
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Guideline
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Prognostic_studies
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Screening_studies
Limite:
Humans
Idioma:
En
Revista:
Diagn Microbiol Infect Dis
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Canadá