Sfp1 regulates transcriptional networks driving cell growth and division through multiple promoter-binding modes.
Genes Dev
; 33(5-6): 288-293, 2019 03 01.
Article
em En
| MEDLINE
| ID: mdl-30804227
ABSTRACT
The yeast Sfp1 protein regulates both cell division and growth but how it coordinates these processes is poorly understood. We demonstrate that Sfp1 directly controls genes required for ribosome production and many other growth-promoting processes. Remarkably, the complete set of Sfp1 target genes is revealed only by a combination of ChIP (chromatin immunoprecipitation) and ChEC (chromatin endogenous cleavage) methods, which uncover two promoter binding modes, one requiring a cofactor and the other a DNA-recognition motif. Glucose-regulated Sfp1 binding at cell cycle "START" genes suggests that Sfp1 controls cell size by coordinating expression of genes implicated in mass accumulation and cell division.
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Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Regulação Fúngica da Expressão Gênica
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Regiões Promotoras Genéticas
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Proteínas de Saccharomyces cerevisiae
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Proteínas de Ligação a DNA
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Redes Reguladoras de Genes
Idioma:
En
Revista:
Genes Dev
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Suíça