Production of 8S- and 10S-hydroxy polyunsaturated fatty acids by recombinant Escherichia coli cells expressing mouse arachidonate 8S-lipoxygenase.
Biotechnol Lett
; 41(4-5): 575-582, 2019 May.
Article
em En
| MEDLINE
| ID: mdl-30825045
ABSTRACT
OBJECTIVE:
To quantitatively hydroxylate 8S- and 10S-positions on polyunsaturated fatty acids by recombinant Escherichia coli cells expressing mouse arachidonate 8S-lipoxygenase (8S-LOX).RESULTS:
Hydroxylated products gained from the conversion of arachidonic acid (204Δ5Z,8Z,11Z,14Z, AA), eicosapentanoic acid (205Δ5Z,8Z,11Z,14Z,17Z, EPA), and (226Δ4Z,7Z,10Z,13Z,16Z,19Z, DHA) by recombinant E. coli cells containing 8S-LOX from mouse were identified as 8S-hydroxy-5,9,11,14(Z,E,Z,Z)-eicosatetranoic acid (8S-HETE), 8S-hydroxy-5,9,11,14,17(Z,E,Z,Z,Z)-eicosapentanoic acid (8S-HEPE), and 10S-hydroxy-4,8,12,14,16,19(Z,E,Z,Z,Z,Z)-docosahexaenoic acid (10S-HDoHE), respectively. Under the optimal hydroxylation conditions of pH 7.5, 30 °C, 5% (v/v) ethanol, 15 g cells l-1, and 5 mM substrate, AA, EPA, and DHA were hydroxylated into 4.37 mM 8S-HETE, 3.77 mM 8S-HEPE, and 3.13 mM 10S-HDoHE for 60, 90, and 60 min, with 87, 75, and 63% molar conversions, respectively.CONCLUSION:
To the best of our knowledge, this is the first quantitatively biotechnological production of 8S-HETE, 8S-HEPE, and 10S-HDoHE.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Araquidonato Lipoxigenases
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Proteínas Recombinantes
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Escherichia coli
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Ácidos Graxos Insaturados
Limite:
Animals
Idioma:
En
Revista:
Biotechnol Lett
Ano de publicação:
2019
Tipo de documento:
Article