Addressing the constraints of Tritrichomonas foetus sample collection in remote areas: lyophilized modified Diamond's media as a substitute for liquid medium.
Parasitology
; 146(9): 1184-1187, 2019 08.
Article
em En
| MEDLINE
| ID: mdl-30859924
ABSTRACT
Bovine trichomoniasis is a notifiable, reproductive disease of cattle caused by the parasite Tritrichomonas foetus. Culturing with modified Diamond's medium (MDM) is required to increase the low number of organisms received from a preputial sample, but is limited in application to remote areas as it requires continuous cold chain storage. This study utilized lyophilization to sustain the viability of MDM during transport in lieu of a continuous cold chain. All lyophilized MDM was able to sustain T. foetus after storage for 42 days at 24 °C, and the results demonstrated that lyophilized MDM was equally as viable as refrigerated liquid MDM. Storage of lyophilized MDM at room temperature for 1 and 7 days did not impact T. foetus yield, both with and without exposure to light. A limitation of the lyophilized MDM was demonstrated with a significant decrease in T. foetus yield when the media was stored at 37 and 58 °C. The lyophilization of MDM provides a robust method of transporting and storing medium prior to reconstitution and inoculation, for use in T. foetus diagnosis and surveillance in remote areas.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Infecções Protozoárias em Animais
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Manejo de Espécimes
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Tricomoníase
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Doenças dos Bovinos
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Tritrichomonas foetus
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Meios de Cultura
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
País/Região como assunto:
Oceania
Idioma:
En
Revista:
Parasitology
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Austrália