lncRNA B4GALT1-AS1 promotes colon cancer cell stemness and migration by recruiting YAP to the nucleus and enhancing YAP transcriptional activity.
J Cell Physiol
; 234(10): 18524-18534, 2019 08.
Article
em En
| MEDLINE
| ID: mdl-30912138
ABSTRACT
Here, an RNA-sequencing assay revealed long noncoding RNAs (lncRNAs) with an ectopic expression between colon cancer (CC) and normal colon epithelial cells, in which lncRNA B4GALT1-AS1 exhibited the highest change. A 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay indicated that B4GALT1-AS1 knockdown had no effect on CC cell viability, however, cell clone formation analysis showed that B4GALT1-AS1 knockdown attenuated the capacity of cell clone formation. Additionally, gene set enrichment analysis of this data set revealed that positive enrichment of stem cell-differentiated signatures and negative embryonic stem cell function and adult tissue stem module were observed in CC cells with B4GALT1-AS1 knockdown. Furthermore, B4GALT1-AS1 knockdown suppressed the stemness-marker expression, the ability of cell spheroid formation, and ALDH1 activity in CC cells. Mechanistically, RNA-sequencing data found that the Hippo pathway in cancer was shown on pathways mostly upregulated by B4GALT1-AS1 knockdown, and B4GALT1-AS1 directly bound to the yes-associated protein (YAP), a downstream executor of the Hippo pathway, and B4GALT1-AS1 knockdown promoted the nuclear cytoplasm translocation of YAP and decreased YAP transcriptional activity. Notably, YAP overexpression attenuated the inhibitory effects mediated by B4GALT1-AS1 knockdown. Our results identify the direct binding of lncRNA B4GALT1-AS1 to YAP, which is responsible for CC cell stemness.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
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Transcrição Gênica
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Células-Tronco Neoplásicas
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Movimento Celular
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Neoplasias do Colo
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Proteínas Adaptadoras de Transdução de Sinal
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RNA Longo não Codificante
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Humans
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Male
Idioma:
En
Revista:
J Cell Physiol
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
China