Determination of genetic effects of SERPINA3 on important growth traits in beef cattle.
Anim Biotechnol
; 31(2): 164-173, 2020 Apr.
Article
em En
| MEDLINE
| ID: mdl-30929567
ABSTRACT
Serine protease inhibitor protein 3 (serpin peptidase inhibitor, clade A, member 3, SERPINA3) is a member of the serpin superfamily, probably related to the yield and quality of muscle. This study focuses on the relationship between SERPINA3 gene polymorphism and growth traits in beef cattle. The study first uses sequencing pooled DNA samples (Pool-Seq), PCR-RFLP and Tetra-primer ARMS-PCR techniques to determine the genetic polymorphisms of SERPINA3 in 765 beef cattle. Then, the polymorphic loci were correlated with the growth characters of cattle. Five SNPs (SNP1A-648G, SNP2T6496A, SNP3G2495A, SNP4T2595A, SNP5A2615G) were found, located in the promoter, introns 5 and SNP 3, 4, 5 were in exons 2, respectively. The observed He was from 0.44 to 0.5, Ne were approaching 2 (1.78 to 2.00). The maximum and minimum PIC (polymorphism information content) values were 0.37 and 0.34, respectively. The association analysis results showed that the SNPs had a significant height in the chest girth and body length. (p < 0.05 or p < 0.01). This will provide important information for the rapid breeding of Chinese yellow cattle and the establishment of a molecular genetic marker database.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Bovinos
/
Serpinas
/
Regulação da Expressão Gênica
Limite:
Animals
Idioma:
En
Revista:
Anim Biotechnol
Assunto da revista:
BIOTECNOLOGIA
Ano de publicação:
2020
Tipo de documento:
Article