The uncharacterized bacterial protein YejG has the same architecture as domain III of elongation factor G.
Proteins
; 87(8): 699-705, 2019 08.
Article
em En
| MEDLINE
| ID: mdl-30958578
ABSTRACT
InterPro family IPR020489 comprises ~1000 uncharacterized bacterial proteins. Previously we showed that overexpressing the Escherichia coli representative of this family, EcYejG, conferred low-level resistance to aminoglycoside antibiotics. In an attempt to shed light on the biochemical function of EcYejG, we have solved its structure using multinuclear solution NMR spectroscopy. The structure most closely resembles that of domain III from elongation factor G (EF-G). EF-G catalyzes ribosomal translocation and mutations in EF-G have also been associated with aminoglycoside resistance. While we were unable to demonstrate a direct interaction between EcYejG and the ribosome, the protein might play a role in translation.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fator G para Elongação de Peptídeos
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Proteínas de Escherichia coli
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Escherichia coli
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Proteins
Assunto da revista:
BIOQUIMICA
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Austrália