Enzymatic transformation of ginsenosides Re, Rg1, and Rf to ginsenosides Rg2 and aglycon PPT by using ß-glucosidase from Thermotoga neapolitana.
Biotechnol Lett
; 41(4-5): 613-623, 2019 May.
Article
em En
| MEDLINE
| ID: mdl-30968346
ABSTRACT
OBJECTIVES:
To enzymatically transform protopanaxatriol by using ß-glucosidase from Thermotoga neapolitana (T. neapolitana) DSM 4359.RESULTS:
Recombinant ß-glucosidase was purified, which molecular weight was about 79.5 kDa. High levels of ginsenoside were obtained using the follow reaction conditions 2 mg ml-1 ginsenoside, 25 U ml-1 enzyme, 85 °C, and pH 5.0. ß-glucosidase converted ginsenoside Re to Rg2, Rf and Rg1 to APPT completely after 3 h under the given conditions, respectively. The enzyme created 1.66 mg ml-1 Rg2 from Re with 553 mg l-1 h-1, 0.85 mg ml-1, and 1.01 mg ml-1 APPT from Rg1 and Rf with 283 and 316 mg l-1 h-1 APPT.CONCLUSIONS:
ß-glucosidase could be useful for the high-yield, rapid, and low-cost preparation of ginsenoside Rg2 from Re, and APPT from the ginsenosides Rg1 and Rf.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Sapogeninas
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Beta-Glucosidase
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Ginsenosídeos
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Thermotoga neapolitana
Idioma:
En
Revista:
Biotechnol Lett
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
China