Your browser doesn't support javascript.
loading
MicroRNA-543 promotes cell invasion and impedes apoptosis in pituitary adenoma via activating the Wnt/ß-catenin pathway by negative regulation of Smad7.
Shen, Da-Wei; Li, Yun-Long; Hou, Yu-Jie; Xu, Zhi-Dan; Li, Yong-Zhe; Chang, Jian-Yong.
Afiliação
  • Shen DW; a Department of Neurosurgery , Yidu Central Hospital of Weifang , Weifang , P. R. China.
  • Li YL; a Department of Neurosurgery , Yidu Central Hospital of Weifang , Weifang , P. R. China.
  • Hou YJ; b Department of Hand and Foot Surgery , Yidu Central Hospital of Weifang , Weifang , P. R. China.
  • Xu ZD; c Pharmacology Teaching and Research Section , Weifang Nursing Vocational College , Weifang , P. R. China.
  • Li YZ; d Department of Neurosurgery , the Second Affiliated Hospital of Harbin Medical University , Harbin , P. R. China.
  • Chang JY; e Department of Neurosurgery , Weifang People's Hospital , Weifang , P. R. China.
Biosci Biotechnol Biochem ; 83(6): 1035-1044, 2019 Jun.
Article em En | MEDLINE | ID: mdl-30973065
ABSTRACT
Pituitary adenomas (PA) are commonly occurring benign neoplasms. Identification of molecular pathway resulting in pituitary tumorigenesis remains challenges in endocrine oncology. The present study was conducted with aim of investigating the role of microRNA-543 (miR-543) in PA development. Up-regulated miR-543 and downregulated Smad7 were observed in PA tissues. Afterwards, the specific mechanism of miR-543 and Smad7 in PA were determined with the use of ectopic expression, depletion and reporter assay experiments. Smad7 was confirmed as a target gene of miR-543. HP75 cells treated with overexpressed miR-543 exhibited increased cell proliferation, migration and invasion, while decreased cell apoptosis as well as expression of Cleaved caspase-3 and Cleaved caspase-8 were observed. Suppression of miR-543 contributed to an opposite trend to the above findings. Based on the findings, the inhibition of miR-543 was found to play a tumor suppressive role in PA through the down-regulation of Wnt/ß-catenin pathway by negatively regulating Smad7.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias Hipofisárias / Adenoma / Apoptose / MicroRNAs / Proteína Smad7 / Beta Catenina / Via de Sinalização Wnt / Invasividade Neoplásica Tipo de estudo: Prognostic_studies Limite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: Biosci Biotechnol Biochem Assunto da revista: BIOQUIMICA / BIOTECNOLOGIA Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias Hipofisárias / Adenoma / Apoptose / MicroRNAs / Proteína Smad7 / Beta Catenina / Via de Sinalização Wnt / Invasividade Neoplásica Tipo de estudo: Prognostic_studies Limite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: Biosci Biotechnol Biochem Assunto da revista: BIOQUIMICA / BIOTECNOLOGIA Ano de publicação: 2019 Tipo de documento: Article