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Cryo-Electron Microscopy Methodology: Current Aspects and Future Directions.
Danev, Radostin; Yanagisawa, Haruaki; Kikkawa, Masahide.
Afiliação
  • Danev R; Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan. Electronic address: rado@m.u-tokyo.ac.jp.
  • Yanagisawa H; Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.
  • Kikkawa M; Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan. Electronic address: mkikkawa@m.u-tokyo.ac.jp.
Trends Biochem Sci ; 44(10): 837-848, 2019 10.
Article em En | MEDLINE | ID: mdl-31078399
Cryo-electron microscopy (cryo-EM) has emerged as a powerful structure determination technique. Its most prolific branch is single particle analysis (SPA), a method being used in a growing number of laboratories worldwide to determine high-resolution protein structures. Cryo-electron tomography (cryo-ET) is another powerful approach that enables visualization of protein complexes in their native cellular environment. Despite the wide-ranging success of cryo-EM, there are many methodological aspects that could be improved. Those include sample preparation, sample screening, data acquisition, image processing, and structure validation. Future developments will increase the reliability and throughput of the technique and reduce the cost and skill level barrier for its adoption.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas / Microscopia Crioeletrônica Idioma: En Revista: Trends Biochem Sci Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas / Microscopia Crioeletrônica Idioma: En Revista: Trends Biochem Sci Ano de publicação: 2019 Tipo de documento: Article