Transcriptional Bursts in a Nonequilibrium Model for Gene Regulation by Supercoiling.
Biophys J
; 117(2): 369-376, 2019 07 23.
Article
em En
| MEDLINE
| ID: mdl-31103229
ABSTRACT
We analyze transcriptional bursting within a stochastic nonequilibrium model, which accounts for the coupling between the dynamics of DNA supercoiling and gene transcription. We find a clear signature of bursty transcription when there is a separation between the timescales of transcription initiation and supercoiling dissipation (the latter may either be diffusive or mediated by topological enzymes, such as type I or type II topoisomerases). In multigenic DNA domains, we observe either bursty transcription or transcription waves; the type of behavior can be selected for by controlling gene activity and orientation. In the bursty phase, the statistics of supercoiling fluctuations at the promoter are markedly non-Gaussian.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Transcrição Gênica
/
DNA Super-Helicoidal
/
Regulação Bacteriana da Expressão Gênica
/
Modelos Genéticos
Idioma:
En
Revista:
Biophys J
Ano de publicação:
2019
Tipo de documento:
Article