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The FANCM-BLM-TOP3A-RMI complex suppresses alternative lengthening of telomeres (ALT).
Lu, Robert; O'Rourke, Julienne J; Sobinoff, Alexander P; Allen, Joshua A M; Nelson, Christopher B; Tomlinson, Christopher G; Lee, Michael; Reddel, Roger R; Deans, Andrew J; Pickett, Hilda A.
Afiliação
  • Lu R; Telomere Length Regulation Unit, Children's Medical Research Institute, Faculty of Medicine and Health, University of Sydney, Westmead, 2145, NSW, Australia.
  • O'Rourke JJ; Genome Stability Unit, St. Vincent's Institute, 9 Princes St, Fitzroy, 3065, VIC, Australia.
  • Sobinoff AP; Department of Medicine (St. Vincent's), University of Melbourne, Parkville, 3052, VIC, Australia.
  • Allen JAM; Telomere Length Regulation Unit, Children's Medical Research Institute, Faculty of Medicine and Health, University of Sydney, Westmead, 2145, NSW, Australia.
  • Nelson CB; Telomere Length Regulation Unit, Children's Medical Research Institute, Faculty of Medicine and Health, University of Sydney, Westmead, 2145, NSW, Australia.
  • Tomlinson CG; Telomere Length Regulation Unit, Children's Medical Research Institute, Faculty of Medicine and Health, University of Sydney, Westmead, 2145, NSW, Australia.
  • Lee M; Telomere Length Regulation Unit, Children's Medical Research Institute, Faculty of Medicine and Health, University of Sydney, Westmead, 2145, NSW, Australia.
  • Reddel RR; Telomere Length Regulation Unit, Children's Medical Research Institute, Faculty of Medicine and Health, University of Sydney, Westmead, 2145, NSW, Australia.
  • Deans AJ; Cancer Research Unit, Children's Medical Research Institute, Faculty of Medicine and Health, University of Sydney, Westmead, 2145, NSW, Australia.
  • Pickett HA; Genome Stability Unit, St. Vincent's Institute, 9 Princes St, Fitzroy, 3065, VIC, Australia. adeans@svi.edu.au.
Nat Commun ; 10(1): 2252, 2019 05 28.
Article em En | MEDLINE | ID: mdl-31138797
The collapse of stalled replication forks is a major driver of genomic instability. Several committed mechanisms exist to resolve replication stress. These pathways are particularly pertinent at telomeres. Cancer cells that use Alternative Lengthening of Telomeres (ALT) display heightened levels of telomere-specific replication stress, and co-opt stalled replication forks as substrates for break-induced telomere synthesis. FANCM is a DNA translocase that can form independent functional interactions with the BLM-TOP3A-RMI (BTR) complex and the Fanconi anemia (FA) core complex. Here, we demonstrate that FANCM depletion provokes ALT activity, evident by increased break-induced telomere synthesis, and the induction of ALT biomarkers. FANCM-mediated attenuation of ALT requires its inherent DNA translocase activity and interaction with the BTR complex, but does not require the FA core complex, indicative of FANCM functioning to restrain excessive ALT activity by ameliorating replication stress at telomeres. Synthetic inhibition of FANCM-BTR complex formation is selectively toxic to ALT cancer cells.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas Nucleares / Proteínas de Transporte / Telômero / DNA Topoisomerases Tipo I / DNA Helicases / Proteínas de Ligação a DNA / RecQ Helicases / Homeostase do Telômero / Neoplasias Limite: Humans Idioma: En Revista: Nat Commun Assunto da revista: BIOLOGIA / CIENCIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Austrália

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas Nucleares / Proteínas de Transporte / Telômero / DNA Topoisomerases Tipo I / DNA Helicases / Proteínas de Ligação a DNA / RecQ Helicases / Homeostase do Telômero / Neoplasias Limite: Humans Idioma: En Revista: Nat Commun Assunto da revista: BIOLOGIA / CIENCIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Austrália