Thioredoxin-1 PEGylation as an In Vitro Method for Drug Target Identification.
Methods Mol Biol
; 1990: 143-149, 2019.
Article
em En
| MEDLINE
| ID: mdl-31148069
ABSTRACT
Redox signaling in the cell, which is essential for cell physiology, involves proteins with free sulfhydryl groups (-SH). Among them, the thioredoxin system plays the most significant role. Many conditions associated with cell malignancies feature the higher expression of thioredoxin, making it an attractive target for new therapeutic drug development. Here we present a simple in vitro model of testing the interaction between thioredoxin and the putative drug. This method is relatively inexpensive and gives the Investigator a first screen of the drug properties, which can be essential for further experimental approaches.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Polietilenoglicóis
/
Compostos de Sulfidrila
/
Tiorredoxinas
/
Técnicas In Vitro
/
Espécies Reativas de Oxigênio
/
Maleimidas
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Estados Unidos