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Myeloid-Derived Suppressor Cells Mediate Immunosuppression After Cardiopulmonary Bypass.
Hübner, Max; Tomasi, Roland; Effinger, David; Wu, Tingting; Klein, Gregor; Bender, Martin; Kilger, Erich; Juchem, Gerd; Schwedhelm, Edzard; Kreth, Simone.
Afiliação
  • Hübner M; Department of Anesthesiology, University Hospital, LMU Munich, Munich, Germany.
  • Tomasi R; Walter-Brendel Center of Experimental Medicine, Faculty of Medicine, LMU Munich, Munich, Germany.
  • Effinger D; Department of Anesthesiology, University Hospital, LMU Munich, Munich, Germany.
  • Wu T; Walter-Brendel Center of Experimental Medicine, Faculty of Medicine, LMU Munich, Munich, Germany.
  • Klein G; Department of Anesthesiology, University Hospital, LMU Munich, Munich, Germany.
  • Bender M; Walter-Brendel Center of Experimental Medicine, Faculty of Medicine, LMU Munich, Munich, Germany.
  • Kilger E; Walter-Brendel Center of Experimental Medicine, Faculty of Medicine, LMU Munich, Munich, Germany.
  • Juchem G; Department of Anesthesiology, University Hospital, LMU Munich, Munich, Germany.
  • Schwedhelm E; Department of Anesthesiology, University Hospital, LMU Munich, Munich, Germany.
  • Kreth S; Department of Anesthesiology, University Hospital, LMU Munich, Munich, Germany.
Crit Care Med ; 47(8): e700-e709, 2019 08.
Article em En | MEDLINE | ID: mdl-31149961
ABSTRACT

OBJECTIVES:

Cardiopulmonary bypass is associated with severe immune dysfunctions. Particularly, a cardiopulmonary bypass-related long-lasting immunosuppressive state predisposes patients to a higher risk of postoperative complications, such as persistent bacterial infections. This study was conducted to elucidate mechanisms of post-cardiopulmonary bypass immunosuppression.

DESIGN:

In vitro studies with human peripheral blood mononuclear cells.

SETTING:

Cardiosurgical ICU, University Research Laboratory. PATIENTS Seventy-one patients undergoing cardiac surgery with cardiopulmonary bypass (enrolled May 2017 to August 2018).

INTERVENTIONS:

Peripheral blood mononuclear cells before and after cardiopulmonary bypass were analyzed for the expression of immunomodulatory cell markers by real-time quantitative reverse transcription polymerase chain reaction. T cell effector functions were determined by enzyme-linked immunosorbent assay, carboxyfluorescein succinimidyl ester staining, and cytotoxicity assays. Expression of cell surface markers was assessed by flow cytometry. CD15 cells were depleted by microbead separation. Serum arginine was measured by mass spectrometry. Patient peripheral blood mononuclear cells were incubated in different arginine concentrations, and T cell functions were tested. MEASUREMENTS AND MAIN

RESULTS:

After cardiopulmonary bypass, peripheral blood mononuclear cells exhibited significantly reduced levels of costimulatory receptors (inducible T-cell costimulator, interleukin 7 receptor), whereas inhibitory receptors (programmed cell death protein 1 and programmed cell death 1 ligand 1) were induced. T cell effector functions (interferon γ secretion, proliferation, and CD8-specific cell lysis) were markedly repressed. In 66 of 71 patients, a not yet described cell population was found, which could be characterized as myeloid-derived suppressor cells. Myeloid-derived suppressor cells are known to impair immune cell functions by expression of the arginine-degrading enzyme arginase-1. Accordingly, we found dramatically increased arginase-1 levels in post-cardiopulmonary bypass peripheral blood mononuclear cells, whereas serum arginine levels were significantly reduced. Depletion of myeloid-derived suppressor cells from post-cardiopulmonary bypass peripheral blood mononuclear cells remarkably improved T cell effector function in vitro. Additionally, in vitro supplementation of arginine enhanced T cell immunocompetence.

CONCLUSIONS:

Cardiopulmonary bypass strongly impairs the adaptive immune system by triggering the accumulation of myeloid-derived suppressor cells. These myeloid-derived suppressor cells induce an immunosuppressive T cell phenotype by increasing serum arginine breakdown. Supplementation with L-arginine may be an effective measure to counteract the onset of immunoparalysis in the setting of cardiopulmonary bypass.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ponte Cardiopulmonar / Imunidade Adaptativa / Células Supressoras Mieloides / Insuficiência Cardíaca / Neutrófilos Limite: Female / Humans / Male / Middle aged Idioma: En Revista: Crit Care Med Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Alemanha

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ponte Cardiopulmonar / Imunidade Adaptativa / Células Supressoras Mieloides / Insuficiência Cardíaca / Neutrófilos Limite: Female / Humans / Male / Middle aged Idioma: En Revista: Crit Care Med Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Alemanha