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Robotic assisted generation of 2'-deoxy-2'-fluoro-modifed RNA aptamers - High performance enabling strategies in aptamer selection.
Breuers, Stefan; Bryant, Laura Lledo; Legen, Tjasa; Mayer, Günter.
Afiliação
  • Breuers S; Life and Medical Sciences Institute, University of Bonn, Gerhard-Domagk-Str. 1, 53121 Bonn, Germany; Center of Aptamer Research & Development, University of Bonn, Gerhard-Domagk-Str. 1, 53121 Bonn, Germany.
  • Bryant LL; Life and Medical Sciences Institute, University of Bonn, Gerhard-Domagk-Str. 1, 53121 Bonn, Germany.
  • Legen T; Life and Medical Sciences Institute, University of Bonn, Gerhard-Domagk-Str. 1, 53121 Bonn, Germany; Center of Aptamer Research & Development, University of Bonn, Gerhard-Domagk-Str. 1, 53121 Bonn, Germany.
  • Mayer G; Life and Medical Sciences Institute, University of Bonn, Gerhard-Domagk-Str. 1, 53121 Bonn, Germany; Center of Aptamer Research & Development, University of Bonn, Gerhard-Domagk-Str. 1, 53121 Bonn, Germany. Electronic address: gmayer@uni-bonn.de.
Methods ; 161: 3-9, 2019 05 15.
Article em En | MEDLINE | ID: mdl-31152781
ABSTRACT
Aptamer selection is a laborious procedure, requiring expertise and significant resources. These characteristics limit the accessibility of researchers to these molecular tools. We describe a selection procedure, making use of a robotic system that allows the fully automated selection of RNA and 2'deoxy-2'-fluoro pyrimidine RNA aptamers. The platform offers a rapid access to aptamers for basic research and development, therefore opening the path to aptamer-based systemic analysis of proteomes in biological settings.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Robótica / RNA / Aptâmeros de Nucleotídeos / Sequenciamento de Nucleotídeos em Larga Escala Limite: Humans Idioma: En Revista: Methods Assunto da revista: BIOQUIMICA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Alemanha
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Robótica / RNA / Aptâmeros de Nucleotídeos / Sequenciamento de Nucleotídeos em Larga Escala Limite: Humans Idioma: En Revista: Methods Assunto da revista: BIOQUIMICA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Alemanha