Glucopyranosyl lipid adjuvant enhances immune response to Ebola virus-like particle vaccine in mice.
Vaccine
; 37(29): 3902-3910, 2019 06 27.
Article
em En
| MEDLINE
| ID: mdl-31174937
ABSTRACT
The identification of adjuvants that promote lasting antigen-specific immunity and augment vaccine efficacy are integral to the development of new protein-based vaccines. The Ebola virus-like particle (VLP) vaccine expressing Ebola virus glycoprotein (GP) and matrix protein (VP40) was used in this study to evaluate the ability of TLR4 agonist glucopyranosyl lipid adjuvant (GLA) formulated in a stable emulsion (SE) to enhance immunogenicity and promote durable protection against mouse-adapted Ebola virus (ma-EBOV). Antibody responses and Ebola-specific T cell responses were evaluated post vaccination. Survival analysis after lethal ma-EBOV challenge was performed 4â¯weeks and 22â¯weeks following final vaccination. GLA-SE enhanced EBOV-specific immunity and resulted in long-term protection against challenge with ma-EBOV infection in a mouse model. Specifically, GLA-SE elicited Th1-skewed antibodies and promoted the generation of EBOV GP-specific polyfunctional T cells. These results provide further support for the utility of TLR4 activating GLA-SE-adjuvanted vaccines.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Adjuvantes Imunológicos
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Vacinas contra Ebola
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Vacinas de Partículas Semelhantes a Vírus
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Glicosídeos
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Lipídeos
Limite:
Animals
Idioma:
En
Revista:
Vaccine
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Estados Unidos