Phospho-mTOR expression in human glioblastoma microglia-macrophage cells.
Neurochem Int
; 129: 104485, 2019 10.
Article
em En
| MEDLINE
| ID: mdl-31195027
ABSTRACT
The glioblastoma (GBM) immune microenvironment is highly heterogeneous, and microglia may represent 30-70% of the entire tumor. However, the role of microglia and other specific immune populations is poorly characterized. Activation of mTOR signaling occurs in numerous human cancers and has roles in microglia-glioma cell interactions. We now show in human tumor specimens (42 patients), that 39% of tumor-associated microglial (TAM) cells express mTOR phosphorylated at Ser-2448; and similar mTOR activation is observed using a human microglia-glioma interaction paradigm. In addition, we confirm previous studies that microglia express urea and ARG1 (taken as M2 marker) in the presence of glioma cells, and this phenotype is down-regulated in the presence of a mTOR inhibitor. These results suggest that mTOR suppression in GBM patients might induce a reduction of the M2 phenotype expression in up to 40% of all TAMs. Since the M2 profile of microglial activation is believed to be associated with tumor progression, reductions in that phenotype may represent an additional anti-tumor mechanism of action of mTOR inhibitors, along with direct anti-proliferative activities.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fosfoproteínas
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Neoplasias Encefálicas
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Microglia
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Glioblastoma
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Serina-Treonina Quinases TOR
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Macrófagos
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Proteínas do Tecido Nervoso
Limite:
Adult
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Aged
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Neurochem Int
Ano de publicação:
2019
Tipo de documento:
Article