Peanut leaf extract has antioxidant and anti-inflammatory activity but no acute toxic effects.
Regul Toxicol Pharmacol
; 107: 104407, 2019 Oct.
Article
em En
| MEDLINE
| ID: mdl-31226392
ABSTRACT
Arachis hypogaea L. (peanut) leaves have been popularly used for the treatment of insomnia and inflammation, but no toxicological study has been performed for this plant preparation. This study aimed to examine the phytochemical composition of peanut leaf hydroalcoholic extract (PLHE) and describe its potential toxic effects and antioxidant and anti-inflammatory properties. The qualitative chemical analysis of PLHE by UHPLC-ESI-HRMS allowed the identification of eight metabolites types (totaling 29 compounds). The 1,1-diphenyl-2-picryl-hydrazyl (DPPH) assay revealed that PLHE had strong antioxidant effects; it also exhibited nitric oxide (NO)-scavenging capacity. Human peripheral blood mononuclear cells (PBMCs) exposed to PLHE showed no reduced cell viability or increased free double-stranded DNA, NO, or reactive species production. PLHE reversed the cytotoxicity, pro-inflammatory (release of interleukin-1ß), and pro-oxidant effects of H2O2 on human PBMCs. Acute PLHE toxicity analysis was performed in vivo using the Organization for Economic Co-operation and Development (OECD) 423 guidelines. PLHE single injection (2000â¯mg/kg, intragastric) did not cause mortality or morbidity or induce changes in hematological or biochemical parameters after 14 days of administration. Thus, PLHE could be a source of bioactive compounds and possesses antioxidant and anti-inflammatory properties without elicitin cytotoxicity or genotoxicity in human PBMCs or acute toxicity in rats.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Arachis
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Extratos Vegetais
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Anti-Inflamatórios
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Antioxidantes
Tipo de estudo:
Guideline
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Qualitative_research
Limite:
Animals
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Female
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Humans
Idioma:
En
Revista:
Regul Toxicol Pharmacol
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Brasil