Thermophoretic trap for single amyloid fibril and protein aggregation studies.
Nat Methods
; 16(7): 611-614, 2019 07.
Article
em En
| MEDLINE
| ID: mdl-31235884
ABSTRACT
The study of the aggregation of soluble proteins into highly ordered, insoluble amyloid fibrils is fundamental for the understanding of neurodegenerative disorders. Here, we present a method for the observation of single amyloid fibrils that allows the investigation of fibril growth, secondary nucleation or fibril breakup that is typically hidden in the average ensemble. Our approach of thermophoretic trapping and rotational diffusion measurements is demonstrated for single Aß40, Aß42 and pyroglutamyl-modified amyloid-ß variant (pGlu3-Aß3-40) amyloid fibrils.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Agregados Proteicos
/
Amiloide
Idioma:
En
Revista:
Nat Methods
Assunto da revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Alemanha