Ligand-observed NMR techniques to probe RNA-small molecule interactions.
Methods Enzymol
; 623: 131-149, 2019.
Article
em En
| MEDLINE
| ID: mdl-31239044
ABSTRACT
A growing understanding of the structure and function of RNA has revealed it as a key regulator of gene expression and disease. A multitude of noncoding functions apart from the central roles of RNA in coding for and facilitating protein biogenesis has stimulated research into RNA as a pharmacological target. Despite many exciting advances, RNA remains an understudied target for small molecules, and techniques to investigate RNA-binding molecules are still emerging. A key stumbling block in this area has been validation of RNA-small molecule interactions. Our laboratory has recently used multiple ligand-observed NMR techniques in this regard, including CPMG and WaterLOGSY. This work describes methods to use these techniques in the context of studying RNA-ligand interactions.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
RNA
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Ressonância Magnética Nuclear Biomolecular
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Bibliotecas de Moléculas Pequenas
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Descoberta de Drogas
Limite:
Humans
Idioma:
En
Revista:
Methods Enzymol
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Estados Unidos