High-Throughput Sequencing-Based Investigation of Viruses in Human Cancers by Multienrichment Approach.
J Infect Dis
; 220(8): 1312-1324, 2019 09 13.
Article
em En
| MEDLINE
| ID: mdl-31253993
ABSTRACT
BACKGROUND:
Viruses and other infectious agents cause more than 15% of human cancer cases. High-throughput sequencing-based studies of virus-cancer associations have mainly focused on cancer transcriptome data.METHODS:
In this study, we applied a diverse selection of presequencing enrichment methods targeting all major viral groups, to characterize the viruses present in 197 samples from 18 sample types of cancerous origin. Using high-throughput sequencing, we generated 710 datasets constituting 57 billion sequencing reads.RESULTS:
Detailed in silico investigation of the viral content, including exclusion of viral artefacts, from de novo assembled contigs and individual sequencing reads yielded a map of the viruses detected. Our data reveal a virome dominated by papillomaviruses, anelloviruses, herpesviruses, and parvoviruses. More than half of the included samples contained 1 or more viruses; however, no link between specific viruses and cancer types were found.CONCLUSIONS:
Our study sheds light on viral presence in cancers and provides highly relevant virome data for future reference.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Metagenoma
/
Sequenciamento de Nucleotídeos em Larga Escala
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Neoplasias
Limite:
Female
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Humans
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Male
Idioma:
En
Revista:
J Infect Dis
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Dinamarca