[Preparation of the mycotoxin ochratoxin A for research and diagnostic purposes]. / Príprava mykotoxinu ochratoxin A k výzkumným a diagnostickým úcelum.
Vet Med (Praha)
; 32(11): 675-80, 1987 Nov.
Article
em Cs
| MEDLINE
| ID: mdl-3125655
ABSTRACT
The following fungal strains were tested for the production of the mycotoxin ochratoxin A Aspergillus melleus (CCM F 802), Aspergillus ochraceus (CCM 8002) and Aspergillus ochraceus (CCM F 803). The strain Aspergillus melleus (CCM F 802) proved to be very suitable for the laboratory preparation of ochratoxin A. The mould was multiplied on a natural substrate for 14 days at the temperature of 28 degrees C and at 100% relative humidity. Ochratoxin A was extracted into organic solvents (chloroform + acetic acid), purified with aqueous alkaline solutions and by preparative thin-layer chromatography. The isolated mycotoxin was identified by physico-chemical methods. The amount of pure ochratoxin A isolated from 600 g of substrate was 305 mg.
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Base de dados:
MEDLINE
Assunto principal:
Ocratoxinas
Tipo de estudo:
Diagnostic_studies
Idioma:
Cs
Revista:
Vet Med (Praha)
Ano de publicação:
1987
Tipo de documento:
Article