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Semiautomated Small-Scale Purification Method for High-Throughput Expression Analysis of Recombinant Proteins.
Kraft, Edward; Franke, Yvonne; Heeringa, Katharine; Shriver, Stephanie; Zilberleyb, Inna; Kugel, Christine; Dela Vega, Trisha; Wong, Athena; Brillantes, Bobby; Ciferri, Claudio; Dutina, George; Lee, Grace; Lehoux, Isabelle; Li, Zhong Rong; Lior-Hoffmann, Lee; Hwang, Jiyoung; Lonergan, Chris; Martin, Lynn; Mortara, Kyle; Nguyen, Lananh; Payandeh, Jian; Perez, Andrew; Sampang, Jun; Singh, Lovejit; Schroeder, Kurt; Tam, Christine; Ti, Shu; Win, Ye Naing; Bowman, Krista.
Afiliação
  • Kraft E; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Franke Y; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Heeringa K; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Shriver S; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Zilberleyb I; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Kugel C; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Dela Vega T; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Wong A; Department of Early Stage Cell Culture, Genentech, Inc., South San Francisco, CA, USA.
  • Brillantes B; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Ciferri C; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Dutina G; Department of Early Stage Cell Culture, Genentech, Inc., South San Francisco, CA, USA.
  • Lee G; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Lehoux I; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Li ZR; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Lior-Hoffmann L; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Hwang J; 23andMe Inc., South San Francisco, CA, USA.
  • Lonergan C; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Martin L; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Mortara K; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Nguyen L; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Payandeh J; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Perez A; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Sampang J; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Singh L; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Schroeder K; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Tam C; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Ti S; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Win YN; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA.
  • Bowman K; Department of Biomolecular Resources, Genentech, Inc., South San Francisco, CA, USA. krafte1@gene.com.
Methods Mol Biol ; 2025: 51-68, 2019.
Article em En | MEDLINE | ID: mdl-31267448
ABSTRACT
The expression analysis of recombinant proteins is a challenging step in any high-throughput protein production pipeline. Often multiple expression systems and a variety of expression construct designs are considered for the production of a protein of interest. There is a strong need to triage constructs rapidly and systematically. This chapter describes a semiautomated method for the simultaneous purification and characterization of proteins expressed from multiple samples of expression cultures from the E. coli, baculovirus expression vector system, and mammalian transient expression systems. This method assists in the selection of the most promising expression construct(s) or the most favorable expression condition(s) to move forward into large-scale protein production.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas Recombinantes Limite: Animals Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas Recombinantes Limite: Animals Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Estados Unidos