Divergent effects of a dnaK mutation on abnormal protein degradation in Escherichia coli.
Mol Microbiol
; 2(1): 31-41, 1988 Jan.
Article
em En
| MEDLINE
| ID: mdl-3130542
ABSTRACT
Escherichia coli bacteria produce at least one 70 kD stress protein, the product of the dnaK gene. We have compared the rates of degradation of different types of abnormal proteins in null Ion E. coli with a partial deletion of the dnaK gene with the rates observed in null Ion dnaK+ cells. We have found that both canavanyl proteins and puromycyl polypeptides are degraded more slowly in the null dnaK mutants than in the dnaK+ strain. However, a temperature-sensitive mutant LacI protein is degraded more rapidly in the null dnaK strain. The stability of this temperature-sensitive LacI protein was also examined in detail under various other conditions.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Proteínas
/
Proteínas de Choque Térmico HSP70
/
Proteínas de Escherichia coli
/
Escherichia coli
/
Genes
/
Genes Bacterianos
/
Proteínas de Choque Térmico
/
Mutação
Idioma:
En
Revista:
Mol Microbiol
Assunto da revista:
BIOLOGIA MOLECULAR
/
MICROBIOLOGIA
Ano de publicação:
1988
Tipo de documento:
Article