Establishment of PITX3-mCherry knock-in reporter human embryonic stem cell line (WAe009-A-23).
Stem Cell Res
; 39: 101499, 2019 08.
Article
em En
| MEDLINE
| ID: mdl-31352199
ABSTRACT
Pituitary homeobox 3 (Pitx3) is a key transcription factor that plays an important role in the development and maintenance of midbrain dopaminergic (mDA) neurons. Here, we established a PITX3-mCherry knock-in reporter human embryonic stem cell (hESC) line using the CRISPR/Cas9 system. PITX3-mCherry hESCs maintained pluripotency marker expression and exhibited the capacity to generate all 3 germ layers and a normal karyotype. After differentiation into mDA neurons, most PITX3 immunoreactivity overlapped with the red fluorescence of mCherry. This reporter cell line may be used to study the development of mDA neurons or to enrich mDA populations for transplantation.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
/
Proteínas de Homeodomínio
/
Células-Tronco Embrionárias
Limite:
Humans
Idioma:
En
Revista:
Stem Cell Res
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Coréia do Sul