[Isolation and identification of endophytic fungi producing harpagoside and harpagide from Scrophularia ningpoensis].
Zhongguo Zhong Yao Za Zhi
; 44(10): 2046-2050, 2019 May.
Article
em Zh
| MEDLINE
| ID: mdl-31355559
ABSTRACT
The endophytic fungi from root,main stem,branch and leaf of Scrophularia ningpoensis were isolated from Zhejiang,whether these strains could yield harpagide or harpagoside were tested by HPLC and LC-MS. According to the morphological characteristic and the similarity of the nucleotide sequence of internal transcribed spacer( ITS) between r DNAs,the strains producing harpagide or harpagoside were identified. The results showed that 210 strains were isolated from the samples,which were classified into 9 orders,13 families and 17 genera by morphological study. Harpagide was detected in endogenous fungi ZJ17 and harpagoside was detected in endogenous fungi ZJ25 by HPLC coupled with LC-MS. ZJ17 was identified as Alternaria alternate and ZJ25 was identified as A.gaisen by its morphology and authenticated by ITS( ITS4 and ITS5 regions and the intervening 5. 8 S rDNA region).
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Piranos
/
Scrophularia
/
Glicosídeos Iridoides
/
Fungos
/
Glicosídeos
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
País/Região como assunto:
Asia
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Assunto da revista:
FARMACOLOGIA
/
TERAPIAS COMPLEMENTARES
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
China