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One-Step Engineering of a Stable, Selectable Marker-Free Autoluminescent Acinetobacter baumannii for Rapid Continuous Assessment of Drug Activity.
Jiang, Huofeng; Gao, Yamin; Zeng, Sheng; Wang, Shuai; Cao, Zhizhong; Tan, Yaoju; Yin, Huancai; Liu, Jianxiong; Zhang, Tianyu.
Afiliação
  • Jiang H; School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, P.R. China.
  • Gao Y; State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong 510530, P.R. China.
  • Zeng S; State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong 510530, P.R. China.
  • Wang S; University of Chinese Academy of Sciences (UCAS), Beijing 100049, P.R. China.
  • Cao Z; State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong 510530, P.R. China.
  • Tan Y; State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong 510530, P.R. China.
  • Yin H; University of Chinese Academy of Sciences (UCAS), Beijing 100049, P.R. China.
  • Liu J; State Key Laboratory of Respiratory Disease, Department of Clinical Laboratory, Guangzhou Chest Hospital, Guangzhou 510095, P.R. China.
  • Zhang T; State Key Laboratory of Respiratory Disease, Department of Clinical Laboratory, Guangzhou Chest Hospital, Guangzhou 510095, P.R. China.
J Microbiol Biotechnol ; 29(9): 1488-1493, 2019 Sep 28.
Article em En | MEDLINE | ID: mdl-31387342
ABSTRACT
The rising cases of multidrug-resistant Acinetobacter baumannii (Ab) and the lack of effective drugs call for quick attention. Here, based on a Tn7 transposon and Xer/dif system, we constructed a stable, selectable marker-free autoluminescent Ab capable of producing visible light without extra substrates. Utilization of this autoluminescent reporter strain has the potential to reduce the time, effort and costs required for the evaluation of activities of anti-Ab drug candidates in vitro.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Testes de Sensibilidade Microbiana / Acinetobacter baumannii / Proteínas Luminescentes / Antibacterianos Idioma: En Revista: J Microbiol Biotechnol Ano de publicação: 2019 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Testes de Sensibilidade Microbiana / Acinetobacter baumannii / Proteínas Luminescentes / Antibacterianos Idioma: En Revista: J Microbiol Biotechnol Ano de publicação: 2019 Tipo de documento: Article