Generation of a single-chain variable fragment phage display antibody library from naïve mice panned against Fasciola hepatica antigens.

Norbury, Luke J; Basalaj, Katarzyna; Baska, Piotr; Zawistowska-Deniziak, Anna; Kalinowska, Alicja; Wilkowski, Przemyslaw; Wesolowska, Agnieszka; Wedrychowicz, Halina

Norbury, Luke J; Basalaj, Katarzyna; Baska, Piotr; Zawistowska-Deniziak, Anna; Kalinowska, Alicja; Wilkowski, Przemyslaw; Wesolowska, Agnieszka; Wedrychowicz, Halina.

Afiliação

Norbury LJ; Witold Stefanski Institute of Parasitology, Polish Academy of Sciences, Twarda 51/55, 00-818, Warsaw, Poland. Electronic address: luke.norbury@twarda.pan.pl.
Basalaj K; Witold Stefanski Institute of Parasitology, Polish Academy of Sciences, Twarda 51/55, 00-818, Warsaw, Poland.
Baska P; Division of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Ciszewskiego 8, 02-786, Warsaw, Poland.
Zawistowska-Deniziak A; Witold Stefanski Institute of Parasitology, Polish Academy of Sciences, Twarda 51/55, 00-818, Warsaw, Poland.
Kalinowska A; Witold Stefanski Institute of Parasitology, Polish Academy of Sciences, Twarda 51/55, 00-818, Warsaw, Poland.
Wilkowski P; Witold Stefanski Institute of Parasitology, Polish Academy of Sciences, Twarda 51/55, 00-818, Warsaw, Poland.
Wesolowska A; Witold Stefanski Institute of Parasitology, Polish Academy of Sciences, Twarda 51/55, 00-818, Warsaw, Poland.
Wedrychowicz H; Witold Stefanski Institute of Parasitology, Polish Academy of Sciences, Twarda 51/55, 00-818, Warsaw, Poland.

Exp Parasitol ; 205: 107737, 2019 Oct.

Article em En | MEDLINE | ID: mdl-31401060

Assuntos

Anticorpos Anti-Helmínticos/metabolismo; Antígenos de Helmintos/imunologia; Fasciola hepatica/imunologia; Anticorpos de Cadeia Única/metabolismo; Animais; Anticorpos Monoclonais/metabolismo; Western Blotting; Catepsinas/imunologia; Técnicas de Visualização da Superfície Celular; Eletroforese em Gel de Ágar; Eletroforese em Gel de Poliacrilamida; Ensaio de Imunoadsorção Enzimática; Camundongos; Modelos Moleculares; Reação em Cadeia da Polimerase

Palavras-chave

Cathepsin; Fasciola hepatica; Phage display; Single-chain variable fragment (scFv)

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Anticorpos Anti-Helmínticos / Fasciola hepatica / Anticorpos de Cadeia Única / Antígenos de Helmintos Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Exp Parasitol Ano de publicação: 2019 Tipo de documento: Article

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