Development of Zinc-Specific iCEST MRI as an Imaging Biomarker for Prostate Cancer.
Angew Chem Int Ed Engl
; 58(43): 15512-15517, 2019 10 21.
Article
em En
| MEDLINE
| ID: mdl-31430007
ABSTRACT
The healthy prostate contains the highest concentration of mobile zinc in the body. As this level decreases dramatically during the initial development of prostate cancer, inâ
vivo detection of prostate zinc content may be applied for diagnosis of prostate cancer. Using 19 F ion chemical exchange saturation transfer magnetic resonance imaging (iCEST MRI) and TF-BAPTA as a fluorinated Zn-binding probe with micromolar sensitivity, we show that iCEST MRI is able to differentiate between normal and malignant prostate cells with a 10-fold difference in contrast following glucose-stimulated zinc secretion inâ
vitro. The iCEST signal decreased in normal prostate cells upon downregulation of the ZIP1 zinc transporter. Inâ
vivo, using an orthotopic prostate cancer mouse model and a transgenic adenocarcinoma of the mouse prostate (TRAMP) model, a gradual decrease of >300 % in iCEST contrast following the transition of normal prostate epithelial cells to cancer cells was detected.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Próstata
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Zinco
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Imageamento por Ressonância Magnética
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Biomarcadores Tumorais
Limite:
Animals
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Humans
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Male
Idioma:
En
Revista:
Angew Chem Int Ed Engl
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Estados Unidos