Comparison and process optimization of PLGA, chitosan and silica nanoparticles for potential oral vaccine delivery.
Ther Deliv
; 10(8): 493-514, 2019 08.
Article
em En
| MEDLINE
| ID: mdl-31496377
Aim: The study compared performance of nanoparticles prepared from synthetic organic, natural organic and inorganic materials as vaccine delivery platforms. Materials & methods: Various formulation (concentration, polymer/silica:surfactant ratio, solvent) and process parameters (homogenization speed and time, ultrasonication) affecting functional performance characteristics of poly(lactic-co-glycolic acid) (PLGA), chitosan and silica-based nanoparticles containing bovine serum albumin were investigated. Nanoparticles were characterized using dynamic light scattering, x-ray diffraction, scanning/transmission electron microscopy, Fourier transform infrared spectroscopy and in vitro protein release. Results: Critical formulation parameters were surfactant concentration (PLGA, silica) and polymer concentration (chitosan). Optimized nanoparticles were spherical in shape with narrow size distribution and size ranges of 100-300 nm (blank) and 150-400 nm (protein loaded). Protein encapsulation efficiency was 26-75% and released within 48 h in a sustained manner. Conclusion: Critical formulation and process parameters affected size of PLGA, chitosan and silica nanoparticles and protein encapsulation, while silica produced the smallest and most stable nanoparticles.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Vacinas
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Dióxido de Silício
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Quitosana
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Nanopartículas
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Copolímero de Ácido Poliláctico e Ácido Poliglicólico
Limite:
Animals
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Humans
Idioma:
En
Revista:
Ther Deliv
Ano de publicação:
2019
Tipo de documento:
Article