Simple differential digital confocal aperture to improve axial response of line-scanning confocal microendoscopes.
Opt Lett
; 44(18): 4519-4522, 2019 Sep 15.
Article
em En
| MEDLINE
| ID: mdl-31517920
ABSTRACT
Line-scanning confocal microendoscopy offers video-rate cellular imaging of scattering tissue with relatively simple hardware, but its axial response is inferior to that of point-scanning systems. Based on Fourier optics theory, we designed differential confocal apertures with a simple subtraction technique to improve the line-scanning sectioning performance. Taking advantage of digital slit apertures on a digital light projector and a CMOS rolling shutter, we demonstrate real-time optical sectioning performance comparable to point scanning in a dual-camera microendoscope (<$6,000). We validate the background rejection capability when imaging porcine columnar epithelium stained with fluorescent contrast agents with different uptake mechanisms and staining properties.
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MEDLINE
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En
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Opt Lett
Ano de publicação:
2019
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Article