The RNA-mediated estrogen receptor α interactome of hormone-dependent human breast cancer cell nuclei.
Sci Data
; 6(1): 173, 2019 09 16.
Article
em En
| MEDLINE
| ID: mdl-31527615
ABSTRACT
Estrogen Receptor alpha (ERα) is a ligand-inducible transcription factor that mediates estrogen signaling in hormone-responsive cells, where it controls key cellular functions by assembling in gene-regulatory multiprotein complexes. For this reason, interaction proteomics has been shown to represent a useful tool to investigate the molecular mechanisms underlying ERα action in target cells. RNAs have emerged as bridging molecules, involved in both assembly and activity of transcription regulatory protein complexes. By applying Tandem Affinity Purification (TAP) coupled to mass spectrometry (MS) before and after RNase digestion in vitro, we generated a dataset of nuclear ERα molecular partners whose association with the receptor involves RNAs. These data provide a useful resource to elucidate the combined role of nuclear RNAs and the proteins identified here in ERα signaling to the genome in breast cancer and other cell types.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Mama
/
RNA Neoplásico
/
Núcleo Celular
/
Receptor alfa de Estrogênio
Tipo de estudo:
Prognostic_studies
Limite:
Female
/
Humans
Idioma:
En
Revista:
Sci Data
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Itália