A rapid colloidal gold-based immunochromatographic strip assay for monitoring nitroxynil in milk.
J Sci Food Agric
; 100(5): 1860-1866, 2020 Mar 30.
Article
em En
| MEDLINE
| ID: mdl-31602650
ABSTRACT
BACKGROUND:
Nitroxynil (NIT) is a veterinary drug against hepatic fluke disease for food-producing cattle and sheep. NIT has a long half-life time in animals since it is highly bound to plasma protein. Therefore NIT possibly remains in animal edible tissues or milk due to drug abuse. In this study, a specific murine monoclonal antibody (mAb) against NIT was prepared and an immunochromatographic strip assay based on the mAb was developed for screening NIT in milk.RESULTS:
The affinity constant of the anti-NIT mAb was 2.93 × 1010 and the anti-NIT mAb had almost no cross-reactivity with other analogs, so that it showed good specificity. The cutoff value of this test strip was considered to be 50 ng mL-1 by the naked eye. When detected by the strip reader, the half maximum inhibition concentration (IC50 ) of the immunoassay strip was calculated to be 5.716 ng mL-1 and the limit of detection (LOD) was 1.146 ng mL-1 . Intra-assay recoveries from 88.80 to 97.13% were obtained, with the highest coefficient of variation (CV) at 9.01%; inter-assay recoveries ranged from 84.60 to 106.87%, with the highest CV at 9.93%.CONCLUSION:
The operative procedure of the proposed method can be completed within 10 min. The strip developed in this study was a practical tool for rapid semiquantitative and quantitative detection of NIT in milk. This study suggested great potential for analytically monitoring NIT in other food samples. © 2019 Society of Chemical Industry.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Imunoensaio
/
Resíduos de Drogas
/
Leite
/
Nitroxinila
/
Antiplatelmínticos
Tipo de estudo:
Evaluation_studies
Limite:
Animals
Idioma:
En
Revista:
J Sci Food Agric
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
China