Icotinib-resistant HCC827 cells produce exosomes with mRNA MET oncogenes and mediate the migration and invasion of NSCLC.
Respir Res
; 20(1): 217, 2019 Oct 12.
Article
em En
| MEDLINE
| ID: mdl-31606039
ABSTRACT
BACKGROUND:
Icotinib has been widely used in patients with non-small cell lung cancer (NSCLC), and have significantly enhanced the overall survival rate of NSCLC patients. However, acquired drug resistance limits its clinical efficacy. Tumor cell-derived exosomes have been reported to participate in various biological processes, including tumor invasion, metastasis and drug resistance. MATERIALS ANDMETHODS:
In the present study, drug resistance was measured by MTT assay. Exosomes were extracted from the cell supernatant using ultracentrifugation and identified by exosomal marker. HCC827 cells were treated with exosomes derived from icotinib-resistant (IR) HCC827 to observe the invasion and migration of parent cells. The expression of exo-mRNA was analyzed by reverse transcription-quantitative polymerase chain reaction (RT-PCR). In addition, 10 exo-mRNAs detecting from the plasma and bronchoalveolar lavage fluid (BALF) of NSCLC patients with icotinib treatment were used to establish a new drug resistant-warning formula.RESULTS:
The oncogene MET into exosomes was identified from icotinib-resistant lung cancer cells, and this was also presented in exosomes in NSCLC patients diagnosed with cancer metastasis after icotinib treatment. The knockdown of MET in exosomes significantly decreased the ability of invasion and migration in HCC827 cells.CONCLUSION:
It was suggested that MET might be specifically package and transferred by exosomes to modify the invasion and migration ability of the surrounding icotinib-sensitive cells.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Oncogenes
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Quinazolinas
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RNA Mensageiro
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Movimento Celular
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Resistencia a Medicamentos Antineoplásicos
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Proteínas Proto-Oncogênicas c-met
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Éteres de Coroa
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Exossomos
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Antineoplásicos Imunológicos
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Invasividade Neoplásica
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Respir Res
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
China